Composite

Part:BBa_K1020004:Design

Designed by: Yue Liu   Group: iGEM13_Tianjin   (2013-08-18)


Biosensor for alkane detection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1028
    Illegal NheI site found at 1051
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 752
    Illegal AgeI site found at 864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1821


Design Notes

We choise a constitutive promoter for AlkR gene and in case of interference, we use a long and strong terminator.


Source

Acinetobacter baylyi ADP1

References