Composite
Part:BBa_K1020004:Design
Designed by: Yue Liu Group: iGEM13_Tianjin (2013-08-18)
Biosensor for alkane detection
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1028
Illegal NheI site found at 1051 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 752
Illegal AgeI site found at 864 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1821
Design Notes
We choise a constitutive promoter for AlkR gene and in case of interference, we use a long and strong terminator.
Source
Acinetobacter baylyi ADP1